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ampka1  (R&D Systems)


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    R&D Systems ampka1
    Ampka1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ampka1/Human%2FMouse%2FRat+AMPK+alpha+1+Antibody/us12478601-700-30-31
    Average 93 stars, based on 16 article reviews
    ampka1 - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Dietary product devoid of at least two non essential amino acids
    Article Snippet: Primary antibodies used were: PHGDH (Sigma Life Science, HPA021241), PSAT1 (Novus Biologicals, NBP1-32920), PSPH (Santa Cruz, sc-98683), Actin I-19-R (Santa-Cruz, sc-1616-R), pERK [Phospho-p44/p42 MAPK (Erk1/2) (Thr202/Tyr204)] (Cell Signalling Technology 9101), AMPKa1 (R&D Systems, AF3197) and Phospho-AMPK T172 (Cell Signalling Technology 2535). qRT-PCR.

    Article Title: Modulating the therapeutic response of tumours to dietary serine and glycine starvation.
    Article Snippet: Primary antibodies used were: PHGDH (Sigma-Aldrich Life Science, HPA021241), PSAT1 (Novus Biologicals, NBP1-32920), PSPH (Santa Cruz, sc-98683), Actin I-19-R (Santa-Cruz, sc-1616-R), pERK (phospho-p44/p42 MAPK (Erk1/2) (Thr202/Tyr204)) (Cell Signalling Technology 9101), AMPKa1 (R&D Systems, AF3197) and phospho-AMPK T172 (Cell Signalling Technology 2535). qRT–PCR.



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    Image Search Results


    MCUR1 downregulation reduces activation of the mTOR pathway through the Ca 2+ -CAMMK2-AMPK axis in human cord blood CD34 + HSPCs (A) The effects of MCUR1 knockdown on mitochondrial ([Ca 2+ ] m ; red) and cytoplasmic ([Ca 2+ ] c ; green) Ca 2+ responses in human cord blood CD34 + HSPCs before and after ionomycin exposure (2.5 μM), under hypoxia (1% O 2 ) and EPO treatment (3 U/mL) for 7 days. (B) Knockdown of AMPKa1 induces mTOR activity and abolishes the decreased mTOR activity by MCUR1 knockdown. (C) The effect of AMPKa1 knockdown on the changes in percentages of basophilic (Bas), polychromatophilic (Pol), and orthochromatic (Ort) cells and erythroblasts and erythrocytes (Ery) by MCUR1 knockdown. Left side shows the representative images. (D) The effect of AMPKa1 knockdown on the decreased fraction of erythroblasts by MCUR1 knockdown. (E) The effect of AMPKa1 knockdown on the decreased fraction of CFU-E population by MCUR1 knockdown. (F) The effects of MCUR1 knockdown on the activity of two upstream kinases of AMPKα, LKB1 and CAMKK2. (G) The effects of inhibition of CAMMK2 activity (by BAPTA-AM, an intracellular Ca 2+ chelator; 1 μM) on the increased AMPKα activity and decreased mTOR activity by MCUR1 knockdown. Data are shown as mean ± SD. Data analyses were conducted by two-tailed t test except where noted otherwise. n.s., not significant. ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

    Journal: Cell Genomics

    Article Title: A highland-adaptation variant near MCUR1 reduces its transcription and attenuates erythrogenesis in Tibetans

    doi: 10.1016/j.xgen.2025.100782

    Figure Lengend Snippet: MCUR1 downregulation reduces activation of the mTOR pathway through the Ca 2+ -CAMMK2-AMPK axis in human cord blood CD34 + HSPCs (A) The effects of MCUR1 knockdown on mitochondrial ([Ca 2+ ] m ; red) and cytoplasmic ([Ca 2+ ] c ; green) Ca 2+ responses in human cord blood CD34 + HSPCs before and after ionomycin exposure (2.5 μM), under hypoxia (1% O 2 ) and EPO treatment (3 U/mL) for 7 days. (B) Knockdown of AMPKa1 induces mTOR activity and abolishes the decreased mTOR activity by MCUR1 knockdown. (C) The effect of AMPKa1 knockdown on the changes in percentages of basophilic (Bas), polychromatophilic (Pol), and orthochromatic (Ort) cells and erythroblasts and erythrocytes (Ery) by MCUR1 knockdown. Left side shows the representative images. (D) The effect of AMPKa1 knockdown on the decreased fraction of erythroblasts by MCUR1 knockdown. (E) The effect of AMPKa1 knockdown on the decreased fraction of CFU-E population by MCUR1 knockdown. (F) The effects of MCUR1 knockdown on the activity of two upstream kinases of AMPKα, LKB1 and CAMKK2. (G) The effects of inhibition of CAMMK2 activity (by BAPTA-AM, an intracellular Ca 2+ chelator; 1 μM) on the increased AMPKα activity and decreased mTOR activity by MCUR1 knockdown. Data are shown as mean ± SD. Data analyses were conducted by two-tailed t test except where noted otherwise. n.s., not significant. ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

    Article Snippet: The small interfering RNAs (siRNAs) targeting MCUR1 , TSC1 , TSC2 , AMPKa1 , CAMKK2 , PU.1 and a non-targeting control siRNA were synthesized by RiboBio (Guangzhou City, China).

    Techniques: Activation Assay, Knockdown, Activity Assay, Inhibition, Two Tailed Test